Polyphasic characterization of rhizobial isolates obtained from different common bean-producing regions

In Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria crucial since biological nitrogen fixation (BNF) an eco-friendly technique. This work aimed to obtain characterize rhizobium isolates based on morpho-physiological, molecular, symbiotic efficiency parameters, using strains SEMIA 4077, 4080, 4088 as references. characteristics colonies, their tolerance salinity temperature, well utilization carbon sources, served basis for morpho-physiological characterization. BOX-PCR, REP-PCR, ERIC-PCR markers were used genotypic Assessment was carried out in greenhouse, determining number nodules (NN), nodule dry weight (NDW), shoot (SDW), total-N (Total-N) accumulation shoot. Among isolates, those exhibiting: neutral culture medium pH, fast growth, colony diameter <2 mm, opaque transparency, homogeneous appearance, cream color predominant. Compared most restrictive factor growth isolates. Most grew sucrose (88.43%) mannitol (87.28%). Genotypic analysis revealed that 90% clustered same group reference strain 4080. TaMsG2R1 BaDeG4R2 showed higher Total-N than should be evaluated future studies under field conditions.